Journal: bioRxiv

Article Title: Inhomogeneous Tau polymerization, core–shell organization, and seed formation during Tau condensate aging

doi: 10.64898/2026.03.18.711671

Figure Lengend Snippet: (A) Schematic of seeding Tau aggregation in Tau biosensor (HEK293 expressing TauRD P301S -CFP) cells by aged Tau/RNA condensates. (B) Example images of Tau biosensor cells seeded, or not, with 24 h-old Tau/RNA condensates. Scale bars = 50 μm. (C) High-resolution imaging and 3D-reconstruction of TauRD P301S -CFP in condensate seeded Tau biosensor cells, with counterstaining of the nuclear envelope by Lamin B1 immunostaining shows subcellular positioning of seeded Tau species: Many small Tau foci form in the cytosol and some at the nuclear envelope, larger cytoplasmic Tau aggregates are positioned close to the nucleus, and some Tau clusters also form in the nucleus. Scale bars = 2 μm. (D) Confocal time course imaging of Tau biosensor cells upon seeding with 24 h-old Tau/RNA condensates. Images show sequential formation of Tau accumulation in the same cell: first, cytosolic Tau foci (CLUS) form, followed by Tau foci at the nuclear envelope (NE), larger cytoplasmic Tau aggregates (CYT) close to the nucleus, and, finally, intranuclear circular Tau aggregates (NUC) can be observed. (E) Quantification Tau accumulation types from time course imaging experiments. For analysis, cytoplasmic CLUS and CYT were combined. n=21 analyzed time course series (z-stack), data shown as mean±SEM, one-way ANOVA with Tukey post-test for percentage at 21 h for each accumulation class. (F) STED microcopy of seeded Tau biosensor cells, counter stained with SiR-tubulin (left panel) or immunostained for Lamin B1 (right panel), showing different Tau accumulation types. Zoom-ins show elongated cytosolic Tau structures adjacent to microtubules (left) and Tau foci at the outer nuclear envelope (right). Position of nuclei are indicated by white stars, inner nuclear envelope-nucleoplasm border is indicated by white, dashed lines. Scale bars = 5 μm in overview and 1 μm in zoom-ins. (G) Principle of CFP lifetime FLIM in Tau biosensor cells expressing TauRD P301S -CFP or TauRD P301S -CFP and TauRD P301S -YFP (TauRD P301S -CFP/YFP). CFP lifetime is quenched by molecular crowding in TauRD P301S -CFP accumulations and by both molecular crowding and Tau-Tau interactions in TauRD P301S -CFP/YFP accumulations. (H) Example images of seeded Tau biosensor cells (top: TauRD P301S -CFP cells; bottom: TauRD P301S -CFP/YFP cells). CFP intensity is shown, as well as CFP lifetime components, fit-free defined based on ROIs in phasor plots), superimposed on CFP intensity. Lifetime components could be defined for free soluble Tau (LT SOL , pink), Tau foci in cytosol (CLUS) and at the nuclear envelope (NE; LT CLUS+NE ), cytosolic (CYT) and nuclear (NUC; LT CYT+NUC ) Tau aggregates, and amyloid-like cytosolic Tau aggregates (AMY; LT AMY ). Scale bars = 5 μm. (I) Lifetimes of Tau accumulation types in TauRD P301S -CFP and TauRD P301S -CFP/YFP accumulations. Data shown as mean±SD, comparison of Tau accumulation types within cell type: one-way ANOVA with Tukey post-test. (J) FRET contribution to CFP lifetime quenching in seeded TauRD P301S -CFP/YFP cells, estimated by subtracting lifetimes of Tau accumulation types measured in TauRD P301S -CFP/YFP cells from that measured in TauRD P301S -CFP cells. % values give the proportion of plotted values to entire CFP lifetime quenching in TauRD P301S -CFP/YFP cells. Data shown as mean±SD. (K) Examples of ODT overlaid with correlative fluorescent image of seeded and unseeded TauRD P301S -CFP/YFP cells. (L) Quantification of densities (mg/ml) determined from RI tomograms for Tau accumulations (CYT, NUC) and subcellular compartments (cytoplasm, nucleoplasm, nuclear envelope, and nucleolus). Note, nuclear envelope density in seeded Tau biosensor cells was determined as proxy for Tau foci at the nuclear envelope. n = 15-66 measurements, box plot shows full data range (Min to Max) with all data points, line indicates median, cross indicates mean. Comparison within aggregate type and subcellular compartments: one-way ANOVA with Tukey post-test, or Student T-test for nuclear envelope.

Article Snippet: HEK293 cells stably expressing the Tau repeat domain (TauRD) containing the frontotemporal dementia (FTD)-mutation P301S and fused to CFP or YFP (Tau biosensor cells; TauRD P301S -CFP/YFP); ATCC #CRL-3275; cells provided by Marc Diamond through Erich Wanker) were grown in 8-well imaging dishes (Ibidi).

Techniques: Expressing, Imaging, Immunostaining, Staining, Comparison

Journal: The Journal of Biological Chemistry

Article Title: C -mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer

doi: 10.1016/j.jbc.2026.111359

Figure Lengend Snippet: TSP1 levels in plasma, ascites, and peritoneal exudate cells from mice with ovarian cancer. HM-1 cells were transplanted into the peritoneal cavities of female mice as in , and samples of plasma, ascites, and PECs were collected at the indicated times post-transplantation. A , TSP1 levels in plasma, ascites, and PECs were examined using an immunoblot analysis with the anti-TSP1 antibody. Total proteins on the membranes were stained as described in the , and used for the loading controls. B , TSP1 levels in plasma and ascites were quantified and shown. NS1, not significant versus plasma (day 0). NS2, not significant versus ascites (day 3). C , TSP1 levels in PECs were quantified and shown. ∗ p < 0.01 versus day 0. TSP1, thrombospondin 1.

Article Snippet: Antibodies against TSP1 (A6.1) (sc-59887) and CFP (A00852-2) were from Santa Cruz Biotechnology Inc. and BOSTER Biological Technology, respectively.

Techniques: Clinical Proteomics, Transplantation Assay, Western Blot, Staining

Journal: The Journal of Biological Chemistry

Article Title: C -mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer

doi: 10.1016/j.jbc.2026.111359

Figure Lengend Snippet: C -Man-Trp production in ex vivo -cultured macrophages and myeloid-derived suppressor cells from peritoneal exudate cells of mice with ovarian cancer. HM-1 cells were transplanted into the peritoneal cavities of female mice as in , and PECs were collected 3 days later. Macrophages and MDSCs were isolated from PECs using a fluorescence-activated cell sorter with several specific antibodies as described in the . A , isolated macrophages (CD11b + F4/80 + ) and MDSCs (CD11b + Gr-1 + ) were cultured ex vivo for 8 h as described in the . C -Man-Trp levels were measured in the cell (Cell) and conditioned medium ( Medium ) at the indicated time points. ∗∗ p < 0.05 versus Time (0 h). B , the level of TSP1 secreted into the conditioned medium was examined using an immunoblot analysis at the indicated time points. Band intensity of TSP1 was quantified as described in the . The change of TSP1 level in medium (ΔTSP1) was calculated based on the TSP1 level in preconditioned medium as described in the . ∗ p < 0.01 versus CD11b + GR-1 + (4 and 8 h). ∗∗ p < 0.05 versus CD11b + F4/80 + (4 h). MDSC, myeloid-derived suppressor cell; TSP1, thrombospondin 1.

Article Snippet: Antibodies against TSP1 (A6.1) (sc-59887) and CFP (A00852-2) were from Santa Cruz Biotechnology Inc. and BOSTER Biological Technology, respectively.

Techniques: Ex Vivo, Cell Culture, Derivative Assay, Isolation, Fluorescence, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: C -mannosyl tryptophan dynamics in a mouse model of the peritoneal dissemination of ovarian cancer

doi: 10.1016/j.jbc.2026.111359

Figure Lengend Snippet: Effects of macrophage depletion on C -Man-Trp levels and the expression of C -Man-Trp metabolism-related molecules in plasma and liver tissues from normal healthy mice. Female mice were intravenously injected with CL or EL. Mice were sacrificed 5 days later, and plasma and liver tissues were collected for analyses. A , C -Man-Trp levels were quantified in plasma. B , CFP levels in plasma was examined using an immunoblot analysis with the antibody against CFP. Total proteins on the membranes were stained as described in the , and used for the loading controls of plasma. Band intensity of CFP was quantified as described in the . n.s., non-specific. C , C -Man-Trp levels were quantified in liver tissue samples. D , Dpy19l1 ( left ), Thbs1 ( middle ), and Cfp ( right ) mRNA levels in liver tissue samples were estimated by RT-qPCR. ∗ p < 0.01, ∗∗ p < 0.05 versus EL. RT-qPCR, reverse-transcription quantitative PCR; CL, clodronate liposomes; EL, empty liposomes; CFP, complement factor properdin .

Article Snippet: Antibodies against TSP1 (A6.1) (sc-59887) and CFP (A00852-2) were from Santa Cruz Biotechnology Inc. and BOSTER Biological Technology, respectively.

Techniques: Expressing, Clinical Proteomics, Injection, Western Blot, Staining, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction, Liposomes